Project number:
2001-620
Project Status:
Completed
Budget expenditure:
$153,194.00
Principal Investigator:
Mark S. Crane
Organisation:
CSIRO Australian Animal Health Laboratory
Project start/end date:
10 Oct 2001
-
31 Oct 2004
Contact:
FRDC
SPECIES
1. Import a range of aquatic birnaviruses which are representative of the most important strains.
2. Evaluate the range of birnavirus specific PCR primers which are currently available for their ability o act as generic primers, capable of detecting any aquatic birnavirus
differentiate pathogenic from non-pathogenic isolates and specifically identify the MH aquatic birnavirus.
3. Following evaluation, select or develop a standard set of PCR primers which are panspecific for aquatic birnaviruses. Validate immunodiagnostic procedures (immunocytochemical and immunohistochemical tests) for the detection and identification of aquatic binaviruses in cell cultures and in histological sections.
4. Develop a set of PCR primers which are specific for the MH aquatic birnavirus isolate.
5. Evaluate the ability of newly developed PCR primers to distinguish between pathogenic aquatic birnaviruses.
6. Evaluate the ability of newly developed PCR primers to, not only identify cell culture isolates of viruses, but also identify birnaviruses in infected fish tissues.
