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Aquaculture Nutrition Subprogram: Technical review, project management and development services.

Project number: 2007-230
Project Status:
Completed
Budget expenditure: $30,903.96
Principal Investigator: Robert van Barneveld
Organisation: Barneveld Nutrition Pty Ltd
Project start/end date: 22 Jul 2008 - 30 Jul 2011
Contact:
FRDC

Need

The need for on-going research into aquaculture nutrition and the need for continued inputs to how this research is conducted to ensure it is completed to the highest possible standard is emphasized by the fact that Australian aquaculture industries have a heavy reliance on imported nutrition technologies, feeds and ingredients for the supply of nutrients to target species. This includes feed manufacturing technologies, ingredients such as bait fish, fish meals, crustacean meals and fish oils, and complete feeds such as those utilised by the prawn industry. Not only does this create issues in relation to imported disease risks, continuity of supply and cost, but it means that many local products are being under utilised.

There is currently renewed interest in establishing a dedicated aquaculture nutrition research program due to the increase in cost of fishmeals and oils, their increasingly limited availability and potential contamination with antibiotic residues such as chloramphenicols if the meals are derived from some aquaculture reared products. In addition, pressure from consumers to limit the use of animal proteins in diets and limit interspecies recycling has applied new pressure to feed manufacturers and increased the need to identify and utilise alternative vegetable protein sources in aquafeeds. The increased value of the Australian dollar has also increased competition from imports and the need for the Australian sector to become increasingly efficient to remain internationally competitive with nutrition playing a key role in their capacity to achieve this.

Objectives

1. Provide expert guidance and direction for aquaculture nutrition research in Australia through maintenance of a strategic plan, provision of technical review services and inputs into project development.
2. Identify nutrition research priorities that are not being addressed through existing portfolios and initiate research projects in this area.
3. Promote capacity building and human capital development through the provision of training and resources in aquaculture nutrition and aquafeed manufacturing.
4. Develop risk management strategies as required for various aquaculture sectors in relation to nutrition to assist the research priority setting process.
5. Improve communication of nutrition research outcomes and current nutrition research between scientists, aquaculturalists, ingredient suppliers and feed manufacturers utilizing workshops, planning meetings and conferences.

Final report

Aquafin CRC - Salmon Aquaculture Subprogram: Facilitation and administration

Project number: 2007-229
Project Status:
Completed
Budget expenditure: $46,143.00
Principal Investigator: Colin Buxton
Organisation: University of Tasmania (UTAS)
Project start/end date: 31 Dec 2006 - 29 Nov 2009
Contact:
FRDC

Need

Like any thriving aquaculture industry, the Atlantic salmon industry needs to continually address biological challenges, market and development opportunities and production efficiencies through a coordinated research effort. This enables the industry to ensure sustainability, profitability and to develop to its full potential. The salmon industry does this through a mix of tactical (projects that addresses issues of immediate concern) and strategic (projects addressing longer-term development issues) research. This is achieved through industry strategic plans and to developing collaborative research projects that address industry bottlenecks and avoid duplication and unnecessary expenditure of a finite research funding base.

The SAS provides the delivery mechanism for this approach by ensuring that research is:
- addressing strategic research priorities,
- outcome focussed,
- supported by industry and management,
- adequately funded,
- properly managed and reviewed,
- properly delivered to stakeholders.

The SAS Aquaculture Implementation Committee is also a fundamental management instrument of the Aquafin CRC, providing a vehicle through which the objectives of both the FRDC and Aquafin CRC are realised.

Objectives

1. To administer and co-ordinate the activities of the SAS.
2. To review project proposals, milestone reports and final reports to ensure stakeholder relevance.
3. To facilitate and chair meetings of the SAS Implementation Committee (SASIC).
4. To ensure appropriate liaison between beneficiaries and research providers in the Salmon Sector and to integrate with other finfish sectors.
5. To communicate findings of the SAS through: a) An annual conference held in conjunction with the Aquafin CRC
b) specialist workshops on topics identified through the course of the program
and c) relevant articles in the CRC newsletters - Aquasplash and Salmon Snapshots

Recfishing Research: National Strategy for Recreational Fisheries Research, Development and Extension

Project number: 2007-227
Project Status:
Completed
Budget expenditure: $286,263.00
Principal Investigator: Bill Sawynok
Organisation: Recfish Australia
Project start/end date: 30 Oct 2007 - 1 Jul 2010
Contact:
FRDC

Need

The need for a more cohesive national approach to recreational fisheries RDE has been recognised by Recfish Australia and FRDC for some years. The first move towards a more cohesive approach occurred in 2001/02 with the establishment of the National Strategy for the Survival of Released Line Caught Fish. For the first time a national approach was developed to a priority issue for the recreational fishing industry and over the following years a new very inclusive approach was taken. Key priorities have been addressed through the strategy, leading to a significant improvement in our knowledge on the survival of released fish and an improvement in the best practices for releasing fish.

In 2005 the FRDC Board recognised the need for a new approach to the broader range of recreational fishing RDE priorities and identified the need for the recreational sector to form a body to take these issues forward. The recreational fishing industry needs information on the social and economic benefits provided by recreational fishing to inform resource sharing and access, counter falling participation rates and facilitate growth of the sector in line with changing community values. Research and promotion of best fishing practices is needed to meet community concerns regarding sustainability and ethical conduct of recreational fishing. Extension of new knowledge needs to be improved as past R&D projects have not adequately used recreational fishers communications networks to transfer new knowledge. This proposal seeks to provide for the formation of that body, to provide for the ongoing planning and coordination needed at a national level in a way that adds value to work undertaken by the States. It will also address FRDC’s requirements for effective extension and adoption of R&D results which is reflected in the adding of E to the traditional R&D.

Objectives

1. Through extensive and ongoing consultation with the recreational sector, develop and maintain a list of current national-level recreational fishing RDE priorities.
2. Work with recreational fishing stakeholders and researchers at all levels to facilitate the development of RDE projects to meet identified priorities.
3. Develop an extension network that will promote the adoption of new knowledge generated by recreational fishing related R&D by decision makers, recreational fishers, and the wider community.
4. Assess the adoption of new knowledge by decision makers, recreational fishers, and the wider community, and identify constraints and barriers to the adoption of new knowledge by these groups.

Aquatic Animal Health Subprogram: rapid strain identification of the bacterial fish pathogen Streptococcus iniae and development of an effective polyvalent vaccine for Australian barramundi

Project number: 2007-226
Project Status:
Completed
Budget expenditure: $254,821.27
Principal Investigator: Andrew C. Barnes
Organisation: University of Queensland (UQ)
Project start/end date: 29 Sep 2007 - 30 Jun 2010
Contact:
FRDC

Need

Streptococcus iniae causes streptococcosis, the most important bacterial disease affecting Australian farmed barramundi. Streptococcosis is a major limiting factor in production of barramundi in freshwater and marine systems. Economic loss occurs through high mortalities, loss of marketable product, lost feed costs and costs of treatment, control, prevention and cleanup. Eradication of the disease from the farm environment is not possible. Vaccination offers an inexpensive, reliable and safe method of preventing the disease; however, current strategies rely on autogenous vaccines, i.e., vaccines prepared from specific strains of the organisms causing disease on a particular farm. Provision for preventative vaccination of stock is currently hampered by the lack of a commercially licensed generic Streptococcus vaccine. Further, the use of autogenous vaccines does not protect the fish from disease caused by newly emergent strains and the reproduction of vaccines in response to each separate occurrence is time consuming. At least seven strains of S.iniae are recognised in Australia, with further strains likely to emerge. Current vaccines are strain specific and therefore cross protection against all isolates in all locations in Australia may not be afforded. It has been demonstrated on at least one occasion in Darwin that a vaccine developed against one strain was ineffective against another. A need exists to: Understand the underlying molecular mechanisms whereby new strains of S.iniae evolve; develop technologies that will provide rapid typing of existing or new strains of S.iniae and; provide a strategy for rapid incorporation of new strains into a polyvalent vaccine for national industry use.

Objectives

1. Characterise the genetic and molecular basis by which biotypes of S.iniae vary in relation to capsular and surface protein antigen presentation and strain variation
2. Develop and implement a rapid antigen typing scheme for S. iniae
3. Establish proof-of-concept for a polyvalent vaccine against all known Australian strains of S.iniae
4. Verify the effectiveness of the vaccine in experimental challenge studies initially using the intraperitoneal injection route of immunisation in comparison with immersion and oral administration.
5. Subject to APVMA approval and time constraints, evaluate the vaccine in field trials on infected farms
6. Transfer rapid identification technology to regional laboratories

Final report

ISBN: 978-0-646-53019-2
Author: Andrew Barnes

Aquatic Animal Health Subprogram: Metazoan parasite survey of selected macro-inshore fish of southeastern Australia, including species of commercial importance

Project number: 2007-225
Project Status:
Completed
Budget expenditure: $135,752.00
Principal Investigator: Kate S. Hutson
Organisation: University of Adelaide
Project start/end date: 28 Aug 2007 - 30 Jun 2010
Contact:
FRDC

Need

Metazoan parasites threaten the sustainability and profitability of the Australian finfish aquaculture industry. It is critical, therefore, to identify local parasite species and determine which are potentially harmful. There have been many studies assessing metazoan parasite fauna of wild fish and fish farmed in sea-cages in the northern hemisphere, but such research is notably absent in the Australia. With current low stocking densities and dispersed farm locations in Australia, the potential for parasitic disease problems in sea-cage aquaculture may not yet be realised.

In the sea-cage environment farmed fish can acquire infections found in local populations of wild fish. The natural occurrence of wild fish near sea-cage farms provides an opportunity for transfer of parasites between wild and farmed populations. The parasite assemblage of the wild fish species and the potential risks of these parasites for sea-cage aquaculture are largely unknown. By gathering biological data about parasites, we will gain a better understanding of how to manage and control them on fish in captivity.

Parasite identification, knowledge of parasite biology, diagnostic tools and how parasites impact on their host is critical for effective parasite management. This project will use a powerful combination of morphological and molecular genetic techniques to provide a comprehensive understanding of copepod, monogenean and trematode parasites infecting selected aquaculture, recreational and commercial finfish species in southeastern Australia, plus barramundi in the northwest.

This research will enable proactive parasite management and rapid identification of pathogenic parasite species. It will also identify appropriate site selection for expansion of the industry away from infection sources. Appropriate husbandry practices and management practices can be put in place to control parasite infections thereby reducing morbidity and mortality in fish stocks.

Objectives

1. To complete a parasite survey of selected finfish species in Australia
2. To provide taxonomic revisions of copepods, monogeneans and trematodes including redescriptions of poorly described taxa and description of new species
3. To provide a host-parasite checklist with biogeographical relevance
4. To use DNA sequences to distinguish blood fluke species and enable diagnostic tools for industry
5. Provide risk analyses for the parasites identified to understand a) the likelihood of parasite establishment and proliferation and b) the consequence of establishment and proliferation for sustainable aquaculture
6. Identify parasites of potential threat to the sustainability of the sea-cage aquaculture industry
7. Develop pathological case studies of parasites of potential threat to aquaculture
8. Identify appropriate husbandry practices to manage and control parasite infections and thereby reduce morbidity and mortality in fish stocks
9. Enable appropriate site selection for expansion of the industry away from infection sources
10. Develop an interactive product (i.e. website) that enables rapid identification of marine parasite species for the public, recreational anglers and sea-cage aquaculture industry

Final report

ISBN: 978-0-646-55210-1
Author: Kate Hutson
Industry
PROJECT NUMBER • 2007-224
PROJECT STATUS:
COMPLETED

Seafood CRC: Increasing the profitability of Penaeus monodon farms via the use of low water exchange, microbial floc production systems at Australian Prawn Farms and at CSIRO

The economic viability of the Australian prawn farming industry is under threat from increased production of farmed prawns in south-east Asia and the substantial decrease in global prawn prices. The Australian industry is experiencing difficulty competing with imports; prawns can be landed...
ORGANISATION:
Australian Prawn Farmers Association (APFA)

Evaluating the Southern Bluefin Tuna cell lines as a platform for testing the effectiveness of antioxidants in preserving flesh quality

Project number: 2007-221
Project Status:
Completed
Budget expenditure: $353,279.00
Principal Investigator: Kathy Schuller
Organisation: Flinders University
Project start/end date: 13 Jul 2007 - 30 Jun 2010
Contact:
FRDC

Need

The vast majority of the Southern Bluefin Tuna (SBT) farmed in South Australia is sold fresh for sashimi production in Japan. Premium prices are obtained for sashimi grade tuna with a high fat content. Unfortunately, fatty fish tissues with their high polyunsaturated fatty acid (PUFA) content are prone to oxidation. This results in poor flesh quality, reduced shelf life and significant trimming of the tuna carcass at the market.

FRDC Project No. 2004/209 investigated the impact of dietary supplements on the flesh quality of farmed SBT. Specifically, it investigated the impact of the dietary antioxidants vitamins C and E and the mineral selenium. Project No. 2004/209 showed that these supplements can extend the shelf life of muscle samples taken from farmed SBT fed diets containing higher than normal concentrations of the antioxidants.

Although Project No. 2004/209 has been very successful, performing diet trials with live SBT is expensive and logistically difficult. It is also time-consuming and the statistical power of the experiments is limited by the fact that it is economically unfeasible to have multiple replicate sea-cages for each dietary treatment. As a result of these constraints, experiments must be repeated over several years to obtain statistically valid results.

Recently, we produced the world’s first SBT cell line and to our knowledge the first cell line for any tuna species. The cell line has the potential to reduce the number of experiments with live SBT and to greatly increase the number of dietary supplements that can be screened in a given period of time. With the cell line, it takes only one week to screen 24 different supplements with 3-4 replicates of each. This compares with years for similar experiments with live SBT.

This project will evaluate the cell line as a platform for testing dietary antioxidant supplements for SBT.

Objectives

1. To adapt existing assays for antioxidant potency from mammalian cell culture systems for use with primary fish cell cultures and immortal fish cell lines.
2. To use the adapted assays with primary fish cell cultures and immortal fish cell lines to screen a broad range of concentrations and combinations of traditional and novel antioxidants for their ability to inhibit lipid oxidation
3. To rank the antioxidants with respect to their antioxidant potency relative to vitamin E, the ‘industry standard’ antioxidant
4. To quantify how the antioxidant concentrations that are effective in primary fish cell cultures and immortal fish cell lines relate to the antioxidant concentrations that need to be added to aquafeeds
5. To undertake a cost benefit analysis of the various antioxidants and antioxidant combinations with respect to their eventual incorporation into feeds
6. To investigate the impact of dietary antioxidants on cellular antioxidants and antioxidant enzymes in primary fish cell cultures and immortal fish cell lines
7. To recommend the best concentrations and combinations of antioxidants to be added to southern bluefin tuna and yellowtail kingfish feeds to maximize fish health and flesh quality

Final report

ISBN: 978-0-646-54976-7
Author: Kathryn Schuller
Industry
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