In November 1992, this project commenced with the development of methods for the evaluation of striped trumpeter (Latris lineata) larvae cultured by the Finfish Development Program (FDP). The first year was spent in the selection of digestive enzymes for investigation and the modification of methods for their detection. In addition to the digestive enzymes selected, lipids and glycogen deposits were also studied to provide an indication of the status of larval energy uptake and storage. The project used the observation and measurement of samples of larvae to provide an assessment of growth, development and the general health of larvae. The methods developed by the project have been detailed in a Technical Report published by the DPI&F.

Preserved samples of larvae were processed, thin-sectioned and stained to provide information on the development of the swimbladder and the gut with its associated organs, as well as the presence of digestive enzymes at different stages of growth. The timing and method of swimbladder inflation, as well as the development of the digestive tract prior to metamorphosis, was investigated, providing valuable basic biological information on the species. This information was used to modify larviculture systems and techniques and resulted in the successful culture of juvenile striped trumpeters, banded morwong and greenback flounder in the 1994 rearing season.