Cumulative data from other parasites suggests that blocking of Aquaporin channels (AQPs) may provide a suitable target for the use of pharmaceuticals to control these diseases. Neoparamoeba share many similarities with other pathogens. However, there is a need to correctly identify the Neoparamoeba AQPs at the molecular level, to undertake preliminary studies to show that these AQPs can be blocked preferentially (without affecting Atlantic salmon AQPs), and to show that block of the parasite AQPs results in inhibition or death of the Neoparamoeba. Central to this work and indeed to any further AGD research is a need to develop low cost, rapid and reliable assays of salmon gill cell – Neoparamoeba interactions. These in-vitro assays would lead to real dollar savings in the search for alternative AGD treatments by providing high throughput capability to assess multiple treatment options (pharmaceuticals, vaccines, chemicals). They provide a means of investigating amoebae – gill interactions as well as differential (pathogen versus host) effects of treatments. Dr Benita Vincent at CSIRO has begun pilot development of these assay systems with good early success, however, further resources are required to see this work to fruition.
Project number:
2010-731
Project Status:
Completed
Budget expenditure:
$53,250.00
Principal Investigator:
Mat T. Cook
Organisation:
CSIRO Oceans and Atmosphere Hobart
Project start/end date:
30 Apr 2010
-
29 Nov 2010
Contact:
FRDC
SPECIES
1. To clone and sequence Neoparamoeba perurans aquaporins
2. To screen a library of known aquaporin blockers against both Neoparaomoeba perurans and Atlantic salmon aquaporins in an in-vitro Xenopus oocyte system
3. To refine and establish routine protocols for an Atlantic salmon gill cell line and in vitro assay for examining amoeba-gill and treatment interactions
4. To take the best identified aquaporin blockers (from objective 2) and test these in vitro to determine if they kill or inhibit Neoparamoeba perurans without affecting Atlantic salmon cells
PROJECT NUMBER
•
2023-071
PROJECT STATUS:
CURRENT
Development of an Experimental Aquaculture Facility (EAF) specific Amoebic Gill Disease (AGD) challenge model that can reliably evaluate treatment interventions to support industry focused AGD studies
Commercial in confidence
ORGANISATION:
Institute for Marine and Antarctic Studies (IMAS) Hobart
PROJECT NUMBER
•
2023-006
PROJECT STATUS:
CURRENT
Assess the future needs of Australia's aquatic animal disease diagnostic system
1. Undertake stakeholder consultation to inform future demands on the diagnostic system over the next 5 to 10 years considering the current diagnostic landscape, and the emergence of new diagnostic needs and technologies.
ORGANISATION:
University of Sydney (USYD)
PROJECT NUMBER
•
2022-135
PROJECT STATUS:
CURRENT
Minor use permit for Chloramine-T in marine and freshwater finfish
1. Obtain data to satisfy identified gaps, and collate available data, to satisfy specified requirements of a minor use permit application for the use of Chloramine-T (N-chloro-4-methylbenzenesulfonamide sodium salt) to treat bacterial or parasite infections in marine and freshwater finfish.
ORGANISATION:
University of Adelaide